Thursday, August 3, 2017

Weeks 7 & 8 - 7/17/2017 & 7/24/2017

Weeks 7 & 8

For the next two weeks, I ran radial arm maze (RAM) trials. A total of 12 trials were run to see if the animals had learned and had improved their times and memory of going into the correct arm. During the first week, some rats still took the full 10 minutes in the maze, but by the second week all of the rats completed the maze in under 10 minutes. While this was a time consuming experiment because I had to record the data myself and there wasn't a computer doing it like for the attention task, I actually enjoyed it. It was fun to see which rats progressed faster and it was also exciting when a rat would finally understand the maze. There were a few rats that went in the maze in a clockwise or counterclockwise motion, whereas some rats just went to different arms but still finished in under 10 minutes. This was also a cool experiment to see because I have seen the Templeton project rats since they were weaned from their mothers, so it has been interesting to see them "grow up."


For the RAM experiment, the rat is put into the black bucket in the center of the maze for about 10 seconds. The bucket is then removed and the rat explores the arms of the maze and hopefully remembers which ones are baited and which ones are not. Any time the rat enters an arm, it is recorded. At the end of the session, either when the rat has entered all of the baited arms or 10 minutes has passed, the rat is returned to its home cage. Then I calculate the working memory errors, reference memory errors, and latency. Working memory errors are how many times the rat entered an arm the was baited past the initial time it entered, and also if it did not enter a baited arm at all. This is because they are only supposed to enter a baited arm once and remember they entered it. For reference memory errors, that is how many times the rat entered the arms that were not baited. And latency is the time divided by how many times the rat entered each arm.


Radial arm maze


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